Ontology highlight
ABSTRACT:
SUBMITTER: Guo Z
PROVIDER: S-EPMC2222722 | biostudies-literature | 2008 Feb
REPOSITORIES: biostudies-literature
Guo Zhefeng Z Cascio Duilio D Hideg Kálmán K Hubbell Wayne L WL
Protein science : a publication of the Protein Society 20071220 2
Site-directed spin labeling provides a means for exploring structure and dynamics in proteins. To interpret the complex EPR spectra that often arise, it is necessary to characterize the rotamers of the spin-labeled side chain and the interactions they make with the local environment in proteins of known structure. For this purpose, crystal structures have been determined for T4 lysozyme bearing a nitroxide side chain (R1) at the solvent-exposed helical sites 41 and 44 in the B helix. These sites ...[more]