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2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48.

ABSTRACT: 2B4 is a cell surface glycoprotein related to CD2 and implicated in the regulation of natural killer and T lymphocyte function. A recombinant protein containing the extracellular region of mouse (m)2B4 attached to avidin-coated fluorescent beads bound to rodent cells, and binding was completely blocked by CD48 monoclonal antibodies (mAbs). Using surface plasmon resonance, we showed that purified soluble mCD48 bound m2B4 with a six- to ninefold higher affinity (Kd approximately 16 microM at 37 degreesC) than its other ligand, CD2. Human CD48 bound human 2B4 with a similar affinity (Kd approximately 8 microM). The finding of an additional ligand for CD48 provides an explanation for distinct functional effects observed on perturbing CD2 and CD48 with mAbs or by genetic manipulation.

SUBMITTER: Brown MH

PROVIDER: S-EPMC2212392 | biostudies-literature | 1998 Dec

REPOSITORIES: biostudies-literature

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2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48.

Brown M H MH Boles K K van der Merwe P A PA Kumar V V Mathew P A PA Barclay A N AN

The Journal of experimental medicine 19981201 11

2B4 is a cell surface glycoprotein related to CD2 and implicated in the regulation of natural killer and T lymphocyte function. A recombinant protein containing the extracellular region of mouse (m)2B4 attached to avidin-coated fluorescent beads bound to rodent cells, and binding was completely blocked by CD48 monoclonal antibodies (mAbs). Using surface plasmon resonance, we showed that purified soluble mCD48 bound m2B4 with a six- to ninefold higher affinity (Kd approximately 16 microM at 37 de ...[more]

PMID: 9841922

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