Ontology highlight
ABSTRACT:
SUBMITTER: Andrecka J
PROVIDER: S-EPMC2224174 | biostudies-literature | 2008 Jan
REPOSITORIES: biostudies-literature
Andrecka Joanna J Lewis Robert R Brückner Florian F Lehmann Elisabeth E Cramer Patrick P Michaelis Jens J
Proceedings of the National Academy of Sciences of the United States of America 20071227 1
Single-pair fluorescence resonance energy transfer was used to track RNA exiting from RNA polymerase II (Pol II) in elongation complexes. Measuring the distance between the RNA 5' end and three known locations within the elongation complex allows us determine its position by means of triangulation. RNA leaves the polymerase active center cleft via the previously proposed exit tunnel and then disengages from the enzyme surface. When the RNA reaches lengths of 26 and 29 nt, its 5' end associates w ...[more]