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RNA Polymerase II cluster dynamics predict mRNA output in living cells.

ABSTRACT: Protein clustering is a hallmark of genome regulation in mammalian cells. However, the dynamic molecular processes involved make it difficult to correlate clustering with functional consequences in vivo. We developed a live-cell super-resolution approach to uncover the correlation between mRNA synthesis and the dynamics of RNA Polymerase II (Pol II) clusters at a gene locus. For endogenous ?-actin genes in mouse embryonic fibroblasts, we observe that short-lived (~8 s) Pol II clusters correlate with basal mRNA output. During serum stimulation, a stereotyped increase in Pol II cluster lifetime correlates with a proportionate increase in the number of mRNAs synthesized. Our findings suggest that transient clustering of Pol II may constitute a pre-transcriptional regulatory event that predictably modulates nascent mRNA output.

SUBMITTER: Cho WK

PROVIDER: S-EPMC4929003 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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RNA Polymerase II cluster dynamics predict mRNA output in living cells.

Cho Won-Ki WK Jayanth Namrata N English Brian P BP Inoue Takuma T Andrews J Owen JO Conway William W Grimm Jonathan B JB Spille Jan-Hendrik JH Lavis Luke D LD Lionnet Timothée T Cisse Ibrahim I II

eLife 20160503

Protein clustering is a hallmark of genome regulation in mammalian cells. However, the dynamic molecular processes involved make it difficult to correlate clustering with functional consequences in vivo. We developed a live-cell super-resolution approach to uncover the correlation between mRNA synthesis and the dynamics of RNA Polymerase II (Pol II) clusters at a gene locus. For endogenous β-actin genes in mouse embryonic fibroblasts, we observe that short-lived (~8 s) Pol II clusters correlate ...[more]

PMID: 27138339

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