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Crystallization and preliminary X-ray crystallographic studies of pig heart carbonyl reductase.

ABSTRACT: Pig heart carbonyl reductase (PHCR), which belongs to the short-chain dehydrogenase/reductase (SDR) family, has been crystallized by the hanging-drop vapour-diffusion method. Two crystal forms (I and II) have been obtained in the presence of NADPH. Form I crystals belong to the tetragonal space group P4(2), with unit-cell parameters a = b = 109.61, c = 94.31 A, and diffract to 1.5 A resolution. Form II crystals belong to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 120.10, c = 147.00 A, and diffract to 2.2 A resolution. Both crystal forms are suitable for X-ray structure analysis at high resolution.

SUBMITTER: Aoki K

PROVIDER: S-EPMC2225176 | biostudies-literature | 2006 Oct

REPOSITORIES: biostudies-literature

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Crystallization and preliminary X-ray crystallographic studies of pig heart carbonyl reductase.

Aoki Ken-ichi K Tanaka Nobutada N Ishikura Shuhei S Araki Naoko N Imamura Yorishige Y Hara Akira A Nakamura Kazuo T KT

Acta crystallographica. Section F, Structural biology and crystallization communications 20060930 Pt 10

Pig heart carbonyl reductase (PHCR), which belongs to the short-chain dehydrogenase/reductase (SDR) family, has been crystallized by the hanging-drop vapour-diffusion method. Two crystal forms (I and II) have been obtained in the presence of NADPH. Form I crystals belong to the tetragonal space group P4(2), with unit-cell parameters a = b = 109.61, c = 94.31 A, and diffract to 1.5 A resolution. Form II crystals belong to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 12 ...[more]

PMID: 17012807

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