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Crystallization and preliminary X-ray crystallographic studies of DnaJ from Streptococcus pneumoniae.


ABSTRACT: DnaJ, cooperating with DnaK and GrpE, promotes the folding of unfolded hydrophobic polypeptides, dissociates protein complexes and translocates protein across membranes. Additionally, DnaJ from Streptococcus pneumoniae (SpDnaJ) is involved in the infectious disease process and is being developed as a potential vaccine to prevent bacterial infection. Here the expression, purification, crystallization and preliminary crystallographic analysis of SpDnaJ are reported. The crystals belong to space groups I222 or I2?2?2? and the diffraction resolution is 3.0?Å with unit-cell parameters a=47.68, b=104.45, c=234.57?Å. The crystal most likely contains one molecule in the asymmetric unit, with a VM value of 3.24?Å3?Da(-1) and a solvent content of 62.1%.

SUBMITTER: Zhao S 

PROVIDER: S-EPMC3606571 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature

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Crystallization and preliminary X-ray crystallographic studies of DnaJ from Streptococcus pneumoniae.

Zhao Shasha S   Jin Li L   Niu Siqiang S   Yang Wei W   Zhang Shaocheng S   Guo Zhen Z   Zhang Hongpeng H   Huang Ailong A   Yin Yibing Y   Wang Deqiang D  

Acta crystallographica. Section F, Structural biology and crystallization communications 20130222 Pt 3


DnaJ, cooperating with DnaK and GrpE, promotes the folding of unfolded hydrophobic polypeptides, dissociates protein complexes and translocates protein across membranes. Additionally, DnaJ from Streptococcus pneumoniae (SpDnaJ) is involved in the infectious disease process and is being developed as a potential vaccine to prevent bacterial infection. Here the expression, purification, crystallization and preliminary crystallographic analysis of SpDnaJ are reported. The crystals belong to space gr  ...[more]

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