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ABSTRACT:
SUBMITTER: van Hellemond EW
PROVIDER: S-EPMC2243256 | biostudies-literature | 2008 Mar
REPOSITORIES: biostudies-literature
van Hellemond Erik W EW van Dijk Marianne M Heuts Dominic P H M DP Janssen Dick B DB Fraaije Marco W MW
Applied microbiology and biotechnology 20080109 3
A gene encoding a putrescine oxidase (PuORh, EC 1.4.3.10) was identified from the genome of Rhodococcus erythropolis NCIMB 11540. The gene was cloned in the pBAD vector and overexpressed at high levels in Escherichia coli. The purified enzyme was shown to be a soluble dimeric flavoprotein consisting of subunits of 50 kDa and contains non-covalently bound flavin adenine dinucleotide as a cofactor. From all substrates, the highest catalytic efficiency was found with putrescine (KM=8.2 microM, kcat ...[more]