Ontology highlight
ABSTRACT:
SUBMITTER: Hu M
PROVIDER: S-EPMC2246057 | biostudies-literature | 2008 Jan
REPOSITORIES: biostudies-literature
Hu Minghui M Qian Luping L Briñas Raymond P RP Lymar Elena S ES Kuznetsova Larisa L Hainfeld James F JF
Journal of structural biology 20071011 1
Cryo-electron microscopy single particle analysis shows limited resolution due to poor alignment precision of noisy images taken under low electron exposure. Certain advantages can be obtained by assembling proteins into two-dimensional (2D) arrays since protein particles are locked into repetitive orientation, thus improving alignment precision. We present a labeling method to prepare protein 2D arrays using gold nanoparticles (NPs) interconnecting genetic tag sites on proteins. As an example, ...[more]