Movies of ice-embedded particles enhance resolution in electron cryo-microscopy.
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ABSTRACT:
SUBMITTER: Campbell MG
PROVIDER: S-EPMC3510009 | biostudies-literature | 2012 Nov
REPOSITORIES: biostudies-literature
Publications
Movies of ice-embedded particles enhance resolution in electron cryo-microscopy.
Campbell Melody G MG Cheng Anchi A Brilot Axel F AF Moeller Arne A Lyumkis Dmitry D Veesler David D Pan Junhua J Harrison Stephen C SC Potter Clinton S CS Carragher Bridget B Grigorieff Nikolaus N
Structure (London, England : 1993) 20120927 11
Low-dose images obtained by electron cryo-microscopy (cryo-EM) are often affected by blurring caused by sample motion during electron beam exposure, degrading signal especially at high resolution. We show here that we can align frames of movies, recorded with a direct electron detector during beam exposure of rotavirus double-layered particles, thereby greatly reducing image blurring caused by beam-induced motion and sample stage instabilities. This procedure increases the efficiency of cryo-EM ...[more]
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