Direct selection and phage display of a Gram-positive secretome.
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ABSTRACT:
SUBMITTER: Jankovic D
PROVIDER: S-EPMC2246268 | biostudies-literature | 2007
REPOSITORIES: biostudies-literature
Publications
Direct selection and phage display of a Gram-positive secretome.
Jankovic Dragana D Collett Michael A MA Lubbers Mark W MW Rakonjac Jasna J
Genome biology 20070101 12
Surface, secreted and transmembrane protein-encoding open reading frames, collectively the secretome, can be identified in bacterial genome sequences using bioinformatics. However, functional analysis of translated secretomes is possible only if many secretome proteins are expressed and purified individually. We have now developed and applied a phage display system for direct selection, identification, expression and purification of bacterial secretome proteins. ...[more]
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