Ontology highlight
ABSTRACT:
SUBMITTER: Leschziner AE
PROVIDER: S-EPMC22612 | biostudies-literature | 1998 Jun
REPOSITORIES: biostudies-literature
Leschziner A E AE Griffin T J TJ Grindley N D ND
Proceedings of the National Academy of Sciences of the United States of America 19980601 13
The Tn552 transposase, a member of the DDE superfamily of transposase and retroviral integrase proteins, has been expressed in soluble form. The purified protein performs concerted strand transfer in vitro, efficiently pairing two preprocessed transposon ends and inserting them into target DNA. For maximum efficiency, both participating DNA ends must contain the two adjacent transposase-binding sites that are the normal constituents of the Tn552 termini. As is the case with transposition in vivo ...[more]