Unknown

Dataset Information

0

PTRF-Cavin, a conserved cytoplasmic protein required for caveola formation and function.


ABSTRACT: Caveolae are abundant cell-surface organelles involved in lipid regulation and endocytosis. We used comparative proteomics to identify PTRF (also called Cav-p60, Cavin) as a putative caveolar coat protein. PTRF-Cavin selectively associates with mature caveolae at the plasma membrane but not Golgi-localized caveolin. In prostate cancer PC3 cells, and during development of zebrafish notochord, lack of PTRF-Cavin expression correlates with lack of caveolae, and caveolin resides on flat plasma membrane. Expression of PTRF-Cavin in PC3 cells is sufficient to cause formation of caveolae. Knockdown of PTRF-Cavin reduces caveolae density, both in mammalian cells and in the zebrafish. Caveolin remains on the plasma membrane in PTRF-Cavin knockdown cells but exhibits increased lateral mobility and accelerated lysosomal degradation. We conclude that PTRF-Cavin is required for caveola formation and sequestration of mobile caveolin into immobile caveolae.

SUBMITTER: Hill MM 

PROVIDER: S-EPMC2265257 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications


Caveolae are abundant cell-surface organelles involved in lipid regulation and endocytosis. We used comparative proteomics to identify PTRF (also called Cav-p60, Cavin) as a putative caveolar coat protein. PTRF-Cavin selectively associates with mature caveolae at the plasma membrane but not Golgi-localized caveolin. In prostate cancer PC3 cells, and during development of zebrafish notochord, lack of PTRF-Cavin expression correlates with lack of caveolae, and caveolin resides on flat plasma membr  ...[more]

Similar Datasets

| S-EPMC2994771 | biostudies-literature
| S-EPMC3193497 | biostudies-literature
| S-EPMC4987143 | biostudies-literature
| S-EPMC2581738 | biostudies-literature
| S-EPMC5017623 | biostudies-literature
| S-EPMC3418245 | biostudies-literature
| S-EPMC3831936 | biostudies-literature
| S-EPMC3190672 | biostudies-literature
| S-EPMC3312891 | biostudies-literature
| S-EPMC6615684 | biostudies-literature