Ontology highlight
ABSTRACT:
SUBMITTER: Klumpp LM
PROVIDER: S-EPMC2265777 | biostudies-literature | 2003 Oct
REPOSITORIES: biostudies-literature
Klumpp Lisa M LM Mackey Andrew T AT Farrell Christopher M CM Rosenberg John M JM Gilbert Susan P SP
The Journal of biological chemistry 20030714 40
Switch I and II are key active site structural elements of kinesins, myosins, and G-proteins. Our analysis of a switch I mutant (R210A) in Drosophila melanogaster kinesin showed a reduction in microtubule affinity, a loss in cooperativity between the motor domains, and an ATP hydrolysis defect leading to aberrant detachment from the microtubule. To investigate the conserved arginine in switch I further, a lysine substitution mutant was generated. The R210K dimeric motor has lost the ability to h ...[more]