Ontology highlight
ABSTRACT:
SUBMITTER: Dombeck DA
PROVIDER: S-EPMC2268027 | biostudies-literature | 2007 Oct
REPOSITORIES: biostudies-literature
Dombeck Daniel A DA Khabbaz Anton N AN Collman Forrest F Adelman Thomas L TL Tank David W DW
Neuron 20071001 1
We report a technique for two-photon fluorescence imaging with cellular resolution in awake, behaving mice with minimal motion artifact. The apparatus combines an upright, table-mounted two-photon microscope with a spherical treadmill consisting of a large, air-supported Styrofoam ball. Mice, with implanted cranial windows, are head restrained under the objective while their limbs rest on the ball's upper surface. Following adaptation to head restraint, mice maneuver on the spherical treadmill a ...[more]