An iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis.
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ABSTRACT:
SUBMITTER: Klinge S
PROVIDER: S-EPMC2268749 | biostudies-literature | 2007 Sep
REPOSITORIES: biostudies-literature
Publications
An iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis.
Klinge Sebastian S Hirst Judy J Maman Joseph D JD Krude Torsten T Pellegrini Luca L
Nature structural & molecular biology 20070819 9
Primases synthesize the RNA primers that are necessary for replication of the parental DNA strands. Here we report that the heterodimeric archaeal/eukaryotic primase is an iron-sulfur (Fe-S) protein. Binding of the Fe-S cluster is mediated by an evolutionarily conserved domain at the C terminus of the large subunit. We further show that the Fe-S domain is essential to the unique ability of the eukaryotic primase to start DNA replication. ...[more]
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