Ontology highlight
ABSTRACT:
SUBMITTER: Grotenbreg GM
PROVIDER: S-EPMC2268777 | biostudies-literature | 2008 Mar
REPOSITORIES: biostudies-literature
Grotenbreg Gijsbert M GM Roan Nadia R NR Guillen Eduardo E Meijers Rob R Wang Jia-Huai JH Bell George W GW Starnbach Michael N MN Ploegh Hidde L HL
Proceedings of the National Academy of Sciences of the United States of America 20080201 10
Class I MHC tetramers allow direct phenotypic identification of CD8(+) T cell populations, but their production remains laborious. A peptide exchange strategy that employs class I MHC products loaded with conditional ligands (caged MHC molecules) provides a fast and straightforward method to obtain diverse arrays of class I MHC tetramers and facilitates CD8(+) T cell epitope discovery. Here, we describe the development of photocleavable analogs of the FAPGNYPAL (SV9) epitope that bind H-2K(b) an ...[more]