Ontology highlight
ABSTRACT:
SUBMITTER: Boutin C
PROVIDER: S-EPMC2270900 | biostudies-literature | 2008
REPOSITORIES: biostudies-literature
Boutin Camille C Diestel Simone S Desoeuvre Angélique A Tiveron Marie-Catherine MC Cremer Harold H
PloS one 20080402 4
Functional gene analysis in vivo represents still a major challenge in biomedical research. Here we present a new method for the efficient introduction of nucleic acids into the postnatal mouse forebrain. We show that intraventricular injection of DNA followed by electroporation induces strong expression of transgenes in radial glia, neuronal precursors and neurons of the olfactory system. We present two proof-of-principle experiments to validate our approach. First, we show that expression of a ...[more]