Dataset Information
Proteomic profiling of endorepellin angiostatic activity on human endothelial cells.
Ontology highlight
ABSTRACT: Background
Endorepellin, the C-terminal domain V of the heparan sulfate proteoglycan perlecan, exhibits powerful and targeted anti-angiogenic activity on endothelial cells. To identify proteins involved with endorepellin anti-angiogenic action, we performed an extensive comparative proteomic analysis between vehicle- and endorepellin-treated human endothelial cells.Results
Proteomic analysis of endorepellin influence on human umbilical vein endothelial cells identified five differentially expressed proteins, three of which (beta-actin, calreticulin, and chaperonin/Hsp60) were down-regulated and two of which (vimentin and the beta subunit of prolyl 4-hydroxylase also known as protein disulfide isomerase) were up-regulated in response to endorepellin treatment-and associated with a fold change (endorepellin/control) /= 2.00, and a statistically significant p-value as determined by Student's t test.Conclusion
The proteins identified represent potential target areas involved with endorepellin anti-angiogenic mechanism of action. Further elucidation as such will ultimately provide useful in utilizing endorepellin as an anti-angiogenic therapy in humans.
SUBMITTER: Zoeller JJ
PROVIDER: S-EPMC2275231 | biostudies-literature | 2008 Feb
REPOSITORIES: biostudies-literature
Publications
Proteomic profiling of endorepellin angiostatic activity on human endothelial cells.
Proteome science 20080212
<h4>Background</h4>Endorepellin, the C-terminal domain V of the heparan sulfate proteoglycan perlecan, exhibits powerful and targeted anti-angiogenic activity on endothelial cells. To identify proteins involved with endorepellin anti-angiogenic action, we performed an extensive comparative proteomic analysis between vehicle- and endorepellin-treated human endothelial cells.<h4>Results</h4>Proteomic analysis of endorepellin influence on human umbilical vein endothelial cells identified five diffe ...[more]