Ontology highlight
ABSTRACT:
SUBMITTER: Pellitteri-Hahn MC
PROVIDER: S-EPMC2814535 | biostudies-literature | 2006 Oct
REPOSITORIES: biostudies-literature
Pellitteri-Hahn M C MC Warren M C MC Didier D N DN Winkler E L EL Mirza S P SP Greene A S AS Olivier M M
Journal of proteome research 20061001 10
Serum albumin contamination of cells cultured in vitro significantly impedes the mass spectrometric analysis of proteins secreted by the cells. Here we report a novel washing and culturing technique for rat vascular endothelial cells that considerably reduces the concentration of the commonly used additive for cell culture, bovine serum albumin (BSA), in the secretome of these cells. Cells are rinsed stringently and cultured for 24 h in serum-free media without appreciably impeding cell growth o ...[more]