Ontology highlight
ABSTRACT:
SUBMITTER: Wales TE
PROVIDER: S-EPMC2279938 | biostudies-literature | 2004 Jul
REPOSITORIES: biostudies-literature
Wales Thomas E TE Richardson Jane S JS Fitzgerald Michael C MC
Protein science : a publication of the Protein Society 20040528 7
The 45-amino acid polypeptide chain of the homodimeric transcriptional repressor, CopG, was chemically synthesized by stepwise solid phase peptide synthesis (SPPS) using a protocol based on Boc-chemistry. The product obtained from the synthesis was readily purified by reversed-phase HPLC to give a good overall yield (21% by weight). Moreover, the synthetic CopG constructs prepared in this work folded into three-dimensional structures similar to the wild-type protein prepared using conventional r ...[more]