Ontology highlight
ABSTRACT:
SUBMITTER: Kilstrup M
PROVIDER: S-EPMC102640 | biostudies-literature | 2000 Jun
REPOSITORIES: biostudies-literature
Kilstrup M M Kristiansen K N KN
Nucleic acids research 20000601 11
In the present report we show that unknown DNA fragments are easily amplified in a single PCR reaction from an oligo-cassette library with a single genome-specific primer in combination with a cassette-specific primer. The novelty of the system, in comparison to the vectorette PCR method, lies in the use of unphosphorylated in contrast with phosphorylated oligo-cassettes in the ligation to the chromosomal DNA fragments. After denaturation of the DNA library, all chromosomal fragments carry a sin ...[more]