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Beta-catenin interacts with MyoD and regulates its transcription activity.

ABSTRACT: Wnt regulation of muscle development is thought to be mediated by the beta-catenin-TCF/LEF-dependent canonical pathway. Here we demonstrate that beta-catenin, not TCF/LEF, is required for muscle differentiation. We showed that beta-catenin interacts directly with MyoD, a basic helix-loop-helix transcription factor essential for muscle differentiation and enhances its binding to E box elements and transcriptional activity. MyoD-mediated transactivation is inhibited in muscle cells when beta-catenin is deficient or the interaction between MyoD and beta-catenin is disrupted. These results demonstrate that beta-catenin is necessary for MyoD function, identifying MyoD as an effector in the Wnt canonical pathway.

SUBMITTER: Kim CH

PROVIDER: S-EPMC2293083 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature

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Beta-catenin interacts with MyoD and regulates its transcription activity.

Kim Chang-Hoon CH Neiswender Hannah H Baik Eun Joo EJ Xiong Wen C WC Mei Lin L

Molecular and cellular biology 20080303 9

Wnt regulation of muscle development is thought to be mediated by the beta-catenin-TCF/LEF-dependent canonical pathway. Here we demonstrate that beta-catenin, not TCF/LEF, is required for muscle differentiation. We showed that beta-catenin interacts directly with MyoD, a basic helix-loop-helix transcription factor essential for muscle differentiation and enhances its binding to E box elements and transcriptional activity. MyoD-mediated transactivation is inhibited in muscle cells when beta-caten ...[more]

PMID: 18316399

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