Ontology highlight
ABSTRACT:
SUBMITTER: Sachdeva S
PROVIDER: S-EPMC2293971 | biostudies-literature | 2008 Apr
REPOSITORIES: biostudies-literature
Sachdeva Sarbjot S Musayev Faik F Alhamadsheh Mamoun M MM Neel Scarsdale J J Tonie Wright H H Reynolds Kevin A KA
Bioorganic chemistry 20071221 2
The dimeric Mycobacterium tuberculosis FabH (mtFabH) catalyses a Claisen-type condensation between an acyl-CoA and malonyl-acyl carrier protein (ACP) to initiate the Type II fatty acid synthase cycle. To analyze the initial covalent acylation of mtFabH with acyl-CoA, we challenged it with mixture of C6-C20 acyl-CoAs and the ESI-MS analysis showed reaction at both subunits and a strict specificity for C12 acyl CoA. Crystallographic and ESI-MS studies of mtFabH with a decyl-CoA disulfide inhibitor ...[more]