High-throughput analysis of spatio-temporal dynamics in Dictyostelium.

ABSTRACT: We demonstrate a time-lapse video approach that allows rapid examination of the spatio-temporal dynamics of Dictyostelium cell populations. Quantitative information was gathered by sampling life histories of more than 2,000 mutant clones from a large mutagenesis collection. Approximately 4% of the clonal lines showed a mutant phenotype at one stage. Many of these could be ordered by clustering into functional groups. The dataset allows one to search and retrieve movies on a gene-by-gene and phenotype-by-phenotype basis.

SUBMITTER: Sawai S 

PROVIDER: S-EPMC2323234 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature