High-throughput analysis of spatio-temporal dynamics in Dictyostelium.
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ABSTRACT:
SUBMITTER: Sawai S
PROVIDER: S-EPMC2323234 | biostudies-literature | 2007
REPOSITORIES: biostudies-literature
Publications
High-throughput analysis of spatio-temporal dynamics in Dictyostelium.
Sawai Satoshi S Guan Xiao-Juan XJ Kuspa Adam A Cox Edward C EC
Genome biology 20070101 7
We demonstrate a time-lapse video approach that allows rapid examination of the spatio-temporal dynamics of Dictyostelium cell populations. Quantitative information was gathered by sampling life histories of more than 2,000 mutant clones from a large mutagenesis collection. Approximately 4% of the clonal lines showed a mutant phenotype at one stage. Many of these could be ordered by clustering into functional groups. The dataset allows one to search and retrieve movies on a gene-by-gene and phen ...[more]
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