Ontology highlight
ABSTRACT:
SUBMITTER: Meyer MM
PROVIDER: S-EPMC2323955 | biostudies-literature | 2003 Aug
REPOSITORIES: biostudies-literature
Meyer Michelle M MM Silberg Jonathan J JJ Voigt Christopher A CA Endelman Jeffrey B JB Mayo Stephen L SL Wang Zhen-Gang ZG Arnold Frances H FH
Protein science : a publication of the Protein Society 20030801 8
The computational algorithm SCHEMA was developed to estimate the disruption caused when amino acid residues that interact in the three-dimensional structure of a protein are inherited from different parents upon recombination. To evaluate how well SCHEMA predicts disruption, we have shuffled the distantly-related beta-lactamases PSE-4 and TEM-1 at 13 sites to create a library of 2(14) (16,384) chimeras and examined which ones retain lactamase function. Sequencing the genes from ampicillin-select ...[more]