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Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase.

ABSTRACT: Sar2676, a pantothenate synthetase with a molecular weight of 31 419 Da from methicillin-resistant Staphylococcus aureus, has been expressed, purified and crystallized at 293 K. The protein crystallizes in a primitive triclinic lattice, with unit-cell parameters a = 45.3, b = 60.5, c = 117.6 A, alpha = 87.2, beta = 81.2, gamma = 68.4 degrees . A complete data set has been collected to 2.3 A resolution at the ESRF. Consideration of the likely solvent content suggested the asymmetric unit to contain four molecules. This has been confirmed by molecular-replacement phasing calculations, which give a solution with four monomers using a monomer of pantothenate synthetase from Escherichia coli (PDB code 1iho), which is 41% identical to Sar2676, as a search model.

SUBMITTER: Seetharamappa J 

PROVIDER: S-EPMC2335074 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature

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Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase.

Seetharamappa Jaldappagari J   Oke Muse M   Liu Huanting H   McMahon Stephen A SA   Johnson Kenneth A KA   Carter Lester L   Dorward Mark M   Zawadzki Michal M   Overton Ian M IM   van Niekirk C A Johannes CA   Graham Shirley S   Botting Catherine H CH   Taylor Garry L GL   White Malcolm F MF   Barton Geoffrey J GJ   Coote Peter J PJ   Naismith James H JH  

Acta crystallographica. Section F, Structural biology and crystallization communications 20070505 Pt 6

Sar2676, a pantothenate synthetase with a molecular weight of 31 419 Da from methicillin-resistant Staphylococcus aureus, has been expressed, purified and crystallized at 293 K. The protein crystallizes in a primitive triclinic lattice, with unit-cell parameters a = 45.3, b = 60.5, c = 117.6 A, alpha = 87.2, beta = 81.2, gamma = 68.4 degrees . A complete data set has been collected to 2.3 A resolution at the ESRF. Consideration of the likely solvent content suggested the asymmetric unit to conta  ...[more]

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