Ontology highlight
ABSTRACT:
SUBMITTER: Kim S
PROVIDER: S-EPMC2335126 | biostudies-literature | 2007 Jul
REPOSITORIES: biostudies-literature
Kim SeungBum S Joo Sangbum S Yoon Hyun C HC Ryu Yeonwoo Y Kim Kyeong Kyu KK Kim T Doohun TD
Acta crystallographica. Section F, Structural biology and crystallization communications 20070611 Pt 7
Ketoprofen, a nonsteroidal anti-inflammatory drug, inhibits the synthesis of prostaglandin. A novel hydrolase (Est25) with high ketoprofen specificity has previously been identified using a metagenomic library from environmental samples. Recombinant Est25 protein with a histidine tag at the N-terminus was expressed in Escherichia coli and purified in a homogenous form. Est25 was crystallized from 2.4 M sodium malonate pH 7.0 and X-ray diffraction data were collected to 1.49 A using synchrotron r ...[more]