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Purification, crystallization and initial crystallographic characterization of brazil-nut allergen Ber e 2.

ABSTRACT: Peanut and tree-nut allergies have attracted considerable attention because of their frequency and their lifelong persistence. Brazil-nut (Bertholletia excelsa) allergies have been well documented and the 11S legumin-like seed storage protein Ber e 2 (excelsin) is one of the two known brazil-nut allergens. In this study, Ber e 2 was extracted from brazil-nut kernels and purified to high purity by crystalline precipitation and gel-filtration chromatography. Well diffracting single crystals were obtained using the hanging-drop vapour-diffusion method. A molecular-replacement structural solution has been obtained. Refinement of the structure is currently under way.

SUBMITTER: Guo F

PROVIDER: S-EPMC2339761 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature

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Purification, crystallization and initial crystallographic characterization of brazil-nut allergen Ber e 2.

Guo Feng F Jin Tengchuan T Howard Andrew A Zhang Yu Zhu YZ

Acta crystallographica. Section F, Structural biology and crystallization communications 20071026 Pt 11

Peanut and tree-nut allergies have attracted considerable attention because of their frequency and their lifelong persistence. Brazil-nut (Bertholletia excelsa) allergies have been well documented and the 11S legumin-like seed storage protein Ber e 2 (excelsin) is one of the two known brazil-nut allergens. In this study, Ber e 2 was extracted from brazil-nut kernels and purified to high purity by crystalline precipitation and gel-filtration chromatography. Well diffracting single crystals were o ...[more]

PMID: 18007055

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