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Purification, crystallization and initial crystallographic characterization of the Ginkgo biloba 11S seed globulin ginnacin.

ABSTRACT: Ginkgo biloba, a well known ;living fossil' native to China, is grown worldwide as an ornamental shade plant. Medicinal and nutritional uses of G. biloba in Asia have a long history. However, ginkgo seed proteins have not been well studied at the biochemical and molecular level. In this study, the G. biloba 11S seed storage protein ginnacin was purified by sequential anion-exchange and gel-filtration chromatography. A crystallization screen was performed and well diffracting single crystals were obtained by the vapor-diffusion method. A molecular-replacement structural solution has been obtained. There are six protomers in an asymmetric unit. Structure refinement is currently in progress.

SUBMITTER: Jin T

PROVIDER: S-EPMC2443976 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature

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Purification, crystallization and initial crystallographic characterization of the Ginkgo biloba 11S seed globulin ginnacin.

Jin Tengchuan T Chen Yu Wei YW Howard Andrew A Zhang Yu Zhu YZ

Acta crystallographica. Section F, Structural biology and crystallization communications 20080628 Pt 7

Ginkgo biloba, a well known ;living fossil' native to China, is grown worldwide as an ornamental shade plant. Medicinal and nutritional uses of G. biloba in Asia have a long history. However, ginkgo seed proteins have not been well studied at the biochemical and molecular level. In this study, the G. biloba 11S seed storage protein ginnacin was purified by sequential anion-exchange and gel-filtration chromatography. A crystallization screen was performed and well diffracting single crystals were ...[more]

PMID: 18607097

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