Unknown

Dataset Information

0

Crystallization and X-ray diffraction analysis of a novel immune-type receptor from Ictalurus punctatus and phasing by selenium anomalous dispersion methods.

ABSTRACT: X-ray diffraction data from crystals of a novel immune-type receptor (NITR10 from the catfish Ictalurus punctatus) were collected to 1.65 A resolution and reduced to the primitive hexagonal lattice. Native and selenomethionine derivatives of NITR10 crystallized under different conditions yielded P3(1)21 crystals. SeMet NITR10 was phased to a correlation coefficient of 0.77 by SAD methods and experimental electron-density maps were calculated to 1.65 A. Five NITR10 molecules are predicted to be present in the asymmetric unit based on the Matthews coefficient.

SUBMITTER: Ostrov DA 

PROVIDER: S-EPMC2344108 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Crystallization and X-ray diffraction analysis of a novel immune-type receptor from Ictalurus punctatus and phasing by selenium anomalous dispersion methods.

Ostrov David A DA   Hernández Prada José A JA   Haire Robert N RN   Cannon John P JP   Magis Andrew T AT   Bailey Kate K   Litman Gary W GW  

Acta crystallographica. Section F, Structural biology and crystallization communications 20071121 Pt 12

X-ray diffraction data from crystals of a novel immune-type receptor (NITR10 from the catfish Ictalurus punctatus) were collected to 1.65 A resolution and reduced to the primitive hexagonal lattice. Native and selenomethionine derivatives of NITR10 crystallized under different conditions yielded P3(1)21 crystals. SeMet NITR10 was phased to a correlation coefficient of 0.77 by SAD methods and experimental electron-density maps were calculated to 1.65 A. Five NITR10 molecules are predicted to be p  ...[more]

Similar Datasets

Unknown Selenium single-wavelength anomalous diffraction de novo phasing using an X-ray-free electron laser.
| S-EPMC5097167 | biostudies-literature
Unknown Crystallization, X-ray diffraction analysis and SIRAS phasing of human ?-L-iduronidase.
| S-EPMC3515382 | biostudies-literature
Unknown Macromolecular structure phasing by neutron anomalous diffraction.
| S-EPMC4980602 | biostudies-literature
Unknown Anomalous high-pressure behavior of amorphous selenium from synchrotron x-ray diffraction and microtomography.
| S-EPMC2533173 | biostudies-literature
Unknown Protein structure determination by single-wavelength anomalous diffraction phasing of X-ray free-electron laser data.
| S-EPMC4856140 | biostudies-literature
Unknown Multi-crystal anomalous diffraction for low-resolution macromolecular phasing.
| S-EPMC3016016 | biostudies-literature
Unknown Bayesian machine learning improves single-wavelength anomalous diffraction phasing.
| S-EPMC6833979 | biostudies-literature
Unknown Purification, crystallization, X-ray diffraction analysis and phasing of an engineered single-chain PvuII restriction endonuclease.
| S-EPMC2339719 | biostudies-literature
Unknown Crystallization, X-ray diffraction analysis and phasing of 17beta-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus.
| S-EPMC1978160 | biostudies-literature
Unknown Multi-wavelength anomalous diffraction de novo phasing using a two-colour X-ray free-electron laser with wide tunability.
| S-EPMC5660077 | biostudies-literature