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Engineered lentivector targeting of dendritic cells for in vivo immunization.


ABSTRACT: We report a method of inducing antigen production in dendritic cells by in vivo targeting with lentiviral vectors that specifically bind to the dendritic cell-surface protein DC-SIGN. To target dendritic cells, we enveloped the lentivector with a viral glycoprotein from Sindbis virus engineered to be DC-SIGN-specific. In vitro, this lentivector specifically transduced dendritic cells and induced dendritic cell maturation. A high frequency (up to 12%) of ovalbumin (OVA)-specific CD8(+) T cells and a significant antibody response were observed 2 weeks after injection of a targeted lentiviral vector encoding an OVA transgene into naive mice. This approach also protected against the growth of OVA-expressing E.G7 tumors and induced regression of established tumors. Thus, lentiviral vectors targeting dendritic cells provide a simple method of producing effective immunity and may provide an alternative route for immunization with protein antigens.

SUBMITTER: Yang L 

PROVIDER: S-EPMC2366162 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature

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Engineered lentivector targeting of dendritic cells for in vivo immunization.

Yang Lili L   Yang Haiguang H   Rideout Kendra K   Cho Taehoon T   Joo Kye Il KI   Ziegler Leslie L   Elliot Abigail A   Walls Anthony A   Yu Dongzi D   Baltimore David D   Wang Pin P  

Nature biotechnology 20080224 3


We report a method of inducing antigen production in dendritic cells by in vivo targeting with lentiviral vectors that specifically bind to the dendritic cell-surface protein DC-SIGN. To target dendritic cells, we enveloped the lentivector with a viral glycoprotein from Sindbis virus engineered to be DC-SIGN-specific. In vitro, this lentivector specifically transduced dendritic cells and induced dendritic cell maturation. A high frequency (up to 12%) of ovalbumin (OVA)-specific CD8(+) T cells an  ...[more]

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