Semi-automated microseeding of nanolitre crystallization experiments.
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ABSTRACT:
SUBMITTER: Walter TS
PROVIDER: S-EPMC2373990 | biostudies-literature | 2008 Jan
REPOSITORIES: biostudies-literature
Publications
Semi-automated microseeding of nanolitre crystallization experiments.
Walter Thomas S TS Mancini Erika J EJ Kadlec Jan J Graham Stephen C SC Assenberg René R Ren Jingshan J Sainsbury Sarah S Owens Raymond J RJ Stuart David I DI Grimes Jonathan M JM Harlos Karl K
Acta crystallographica. Section F, Structural biology and crystallization communications 20071220 Pt 1
A simple semi-automated microseeding procedure for nanolitre crystallization experiments is described. Firstly, a microseed stock solution is made from microcrystals using a Teflon bead. A dilution series of this microseed stock is then prepared and dispensed as 100 nl droplets into 96-well crystallization plates, facilitating the incorporation of seeding into high-throughput crystallization pipelines. This basic microseeding procedure has been modified to include additive-screening and cross-se ...[more]
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