An extended hydrophobic core induces EF-hand swapping.
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ABSTRACT:
SUBMITTER: Hakansson M
PROVIDER: S-EPMC2374197 | biostudies-literature | 2001 May
REPOSITORIES: biostudies-literature
Publications
An extended hydrophobic core induces EF-hand swapping.
Håkansson M M Svensson A A Fast J J Linse S S
Protein science : a publication of the Protein Society 20010501 5
The structure of calbindin D(9k) with two substitutions was determined by X-ray crystallography at 1.8-A resolution. Unlike wild-type calbindin D(9k), which is a monomeric protein with two EF-hands, the structure of the mutated calbindin D(9k) reveals an intertwined dimer. In the dimer, two EF-hands of the monomers have exchanged places, and thus a 3D domain-swapped dimer has been formed. EF-hand I of molecule A is packed toward EF-hand II of molecule B and vice versa. The formation of a hydroph ...[more]
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