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Structure of the SH3 domain of rat endophilin A2.

ABSTRACT: The crystal structure of the SH3 domain of rat endophilin A2 has been determined by the multiwavelength anomalous dispersion method and refined at a resolution of 1.70 A to R and R(free) values of 0.196 and 0.217, respectively. The structure adheres to the canonical SH3-domain fold and is highly similar to those of the corresponding domains of endophilins A1 and A3. An intermolecular packing interaction between two molecules in the lattice exploits features that are commonly observed in SH3-domain ligand recognition, including the insertion of a proline side chain into the ligand-binding groove of the protein and the recognition of a basic residue by a cluster of acidic side chains on the RT loop.

SUBMITTER: Loll PJ

PROVIDER: S-EPMC2374248 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature

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Structure of the SH3 domain of rat endophilin A2.

Loll Patrick J PJ Swain Evelyn E Chen Yuan Y Turner Brian T BT Zhang Ji-fang JF

Acta crystallographica. Section F, Structural biology and crystallization communications 20080329 Pt 4

The crystal structure of the SH3 domain of rat endophilin A2 has been determined by the multiwavelength anomalous dispersion method and refined at a resolution of 1.70 A to R and R(free) values of 0.196 and 0.217, respectively. The structure adheres to the canonical SH3-domain fold and is highly similar to those of the corresponding domains of endophilins A1 and A3. An intermolecular packing interaction between two molecules in the lattice exploits features that are commonly observed in SH3-doma ...[more]

PMID: 18391417

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Vesicle uncoating regulated by SH3-SH3 domain-mediated complex formation between endophilin and intersectin at synapses.

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Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis.

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Genome-wide gene expression analysis on tibialis anterior muscle from nebulin SH3 domain deleted (Neb∆SH3) mice

Project description:Genome-wide gene expression analysis on tibialis anterior muscle from 2-month-old nebulin SH3 domain deleted (Neb∆SH3) mice compared to wildtype.

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