High-resolution 3D structure determination of kaliotoxin by solid-state NMR spectroscopy.

ABSTRACT: High-resolution solid-state NMR spectroscopy can provide structural information of proteins that cannot be studied by X-ray crystallography or solution NMR spectroscopy. Here we demonstrate that it is possible to determine a protein structure by solid-state NMR to a resolution comparable to that by solution NMR. Using an iterative assignment and structure calculation protocol, a large number of distance restraints was extracted from (1)H/(1)H mixing experiments recorded on a single uniformly labeled sample under magic angle spinning conditions. The calculated structure has a coordinate precision of 0.6 A and 1.3 A for the backbone and side chain heavy atoms, respectively, and deviates from the structure observed in solution. The approach is expected to be applicable to larger systems enabl

SUBMITTER: Korukottu J 

PROVIDER: S-EPMC2387072 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature