Ontology highlight
ABSTRACT:
SUBMITTER: Nieuwkoop AJ
PROVIDER: S-EPMC2895330 | biostudies-literature | 2010 Jun
REPOSITORIES: biostudies-literature
Nieuwkoop Andrew J AJ Rienstra Chad M CM
Journal of the American Chemical Society 20100601 22
We demonstrate that 3D Z-filtered TEDOR experiments, when performed on mixtures of isotopically labeled protein samples, report on site-specific intermolecular distance restraints. These data sets can be leveraged to perform rigorous structure calculations of the protein interface. In the example demonstrated here, we determine the packing arrangement of our nanocrystalline GB1 preparation to be consistent with the trigonal form as determined by X-ray diffraction. This represents an important pr ...[more]