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UV laser mediated cell selective destruction by confocal microscopy.

ABSTRACT: Analysis of cell-cell interactions, cell function and cell lineages greatly benefits selective destruction techniques, which, at present, rely on dedicated, high energy, pulsed lasers and are limited to cells that are detectable by conventional microscopy. We present here a high resolution/sensitivity technique based on confocal microscopy and relying on commonly used UV lasers. Coupling this technique with time-lapse enables the destruction and following of any cell(s) in any pattern(s) in living animals as well as in cell culture systems.

SUBMITTER: Soustelle L

PROVIDER: S-EPMC2387153 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature

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UV laser mediated cell selective destruction by confocal microscopy.

Soustelle Laurent L Aigouy Benoît B Asensio Marie-Laure ML Giangrande Angela A

Neural development 20080428

Analysis of cell-cell interactions, cell function and cell lineages greatly benefits selective destruction techniques, which, at present, rely on dedicated, high energy, pulsed lasers and are limited to cells that are detectable by conventional microscopy. We present here a high resolution/sensitivity technique based on confocal microscopy and relying on commonly used UV lasers. Coupling this technique with time-lapse enables the destruction and following of any cell(s) in any pattern(s) in livi ...[more]

PMID: 18442390

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