Dataset Information

Longitudinal confocal microscopy imaging of solid tumor destruction following adoptive T cell transfer.

ABSTRACT: A fluorescence-based, high-resolution imaging approach was used to visualize longitudinally the cellular events unfolding during T cell-mediated tumor destruction. The dynamic interplay of T cells, cancer cells, cancer antigen loss variants, and stromal cells-all color-coded in vivo-was analyzed in established, solid tumors that had developed behind windows implanted on the backs of mice. Events could be followed repeatedly within precisely the same tumor region-before, during and after adoptive T cell therapy-thereby enabling for the first time a longitudinal in vivo evaluation of protracted events, an analysis not possible with terminal imaging of surgically exposed tumors. T cell infiltration, stromal interactions, and vessel destruction, as well as the functional consequences thereof, including the elimination of cancer cells and cancer cell variants were studied. Minimal perivascular T cell infiltrates initiated vascular destruction inside the tumor mass eventually leading to macroscopic central tumor necrosis. Prolonged engagement of T cells with tumor antigen-crosspresenting stromal cells correlated with high IFN? cytokine release and bystander elimination of antigen-negative cancer cells. The high-resolution, longitudinal, in vivo imaging approach described here will help to further a better mechanistic understanding of tumor eradication by T cells and other anti-cancer therapies.

SUBMITTER: Schietinger A

PROVIDER: S-EPMC3895414 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Longitudinal confocal microscopy imaging of solid tumor destruction following adoptive T cell transfer.

Schietinger Andrea A Arina Ainhoa A Liu Rebecca B RB Wells Sam S Huang Jianhua J Engels Boris B Bindokas Vytas V Bartkowiak Todd T Lee David D Herrmann Andreas A Piston David W DW Pittet Mikael J MJ Lin P Charles PC Zal Tomasz T Schreiber Hans H

Oncoimmunology 20131104 11

A fluorescence-based, high-resolution imaging approach was used to visualize longitudinally the cellular events unfolding during T cell-mediated tumor destruction. The dynamic interplay of T cells, cancer cells, cancer antigen loss variants, and stromal cells-all color-coded in vivo-was analyzed in established, solid tumors that had developed behind windows implanted on the backs of mice. Events could be followed repeatedly within precisely the same tumor region-before, during and after adoptive ...[more]

PMID: 24482750

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Dataset Information

Longitudinal confocal microscopy imaging of solid tumor destruction following adoptive T cell transfer.

Publications

Longitudinal confocal microscopy imaging of solid tumor destruction following adoptive T cell transfer.

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