Ontology highlight
ABSTRACT:
SUBMITTER: Smailus DE
PROVIDER: S-EPMC2398714 | biostudies-literature | 2007 Aug
REPOSITORIES: biostudies-literature
Smailus Duane E DE Warren Rene L RL Holt Robert A RA
Systems and synthetic biology 20070801 3
Methods for constructing large contiguous segments of DNA will be enabling for Synthetic Biology, where the assembly of genes encoding circuits, biosynthetic pathways or even whole microbial organisms is of interest. Currently, in vitro approaches to DNA synthesis are adequate for generating DNAs that are up to 10s of kbp in length, and in vivo recombination strategies are more suitable for building DNA constructs that are 100 kbp or larger. We have developed a vector system for efficient assemb ...[more]