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Iterative plug-and-play methodology for constructing and modifying synthetic gene networks.

ABSTRACT: We present a methodology for the design, construction and modification of synthetic gene networks. This method emphasizes post-assembly modification of constructs based on network behavior, thus facilitating iterative design strategies and rapid tuning and repurposing of gene networks. The ease of post-construction modification afforded by this approach and the ever-increasing repository of components within the framework will help accelerate the development of functional genetic circuits for synthetic biology.

SUBMITTER: Litcofsky KD

PROVIDER: S-EPMC3492501 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature

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Iterative plug-and-play methodology for constructing and modifying synthetic gene networks.

Litcofsky Kevin D KD Afeyan Raffi B RB Krom Russell J RJ Khalil Ahmad S AS Collins James J JJ

Nature methods 20121007 11

We present a methodology for the design, construction and modification of synthetic gene networks. This method emphasizes post-assembly modification of constructs based on network behavior, thus facilitating iterative design strategies and rapid tuning and repurposing of gene networks. The ease of post-construction modification afforded by this approach and the ever-increasing repository of components within the framework will help accelerate the development of functional genetic circuits for sy ...[more]

PMID: 23042452

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