Ontology highlight
ABSTRACT:
SUBMITTER: Faye T
PROVIDER: S-EPMC2423041 | biostudies-literature | 2008 Jun
REPOSITORIES: biostudies-literature
Applied and environmental microbiology 20080418 11
A beta-galactosidase reporter system for the analysis of promoter elements in Propionibacterium freudenreichii was designed. The pTD210 in vivo reporter vector was constructed using a promoterless lacZ gene from Bifidobacterium longum cloned into the pAMT1 plasmid. The utility of the pTD210 reporter vector was demonstrated by an investigation of six predicted promoters in P. freudenreichii. The system produced accurate and reproducible measurements that facilitated both promoter identification a ...[more]