Epitope tagging of endogenous proteins for genome-wide ChIP-chip studies.
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ABSTRACT:
SUBMITTER: Zhang X
PROVIDER: S-EPMC2435063 | biostudies-literature | 2008 Feb
REPOSITORIES: biostudies-literature
Publications
Epitope tagging of endogenous proteins for genome-wide ChIP-chip studies.
Zhang Xiaodong X Guo Chunguang C Chen Yueting Y Shulha Hennady P HP Schnetz Michael P MP LaFramboise Thomas T Bartels Cynthia F CF Markowitz Sanford S Weng Zhiping Z Scacheri Peter C PC Wang Zhenghe Z
Nature methods 20080106 2
We developed a strategy to introduce epitope tag-encoding DNA into endogenous loci by homologous recombination-mediated 'knock-in'. The tagging method is straightforward, can be applied to many loci and several human somatic cell lines, and can facilitate many functional analyses including western blot, immunoprecipitation, immunofluorescence and chromatin immunoprecipitation-microarray (ChIP-chip). The knock-in approach provides a general solution for the study of proteins to which antibodies a ...[more]
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