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Mosquito densonucleosis virus non-structural protein NS2 is necessary for a productive infection.

ABSTRACT: Mosquito densonucleosis viruses synthesize two non-structural proteins, NS1 and NS2. While NS1 has been studied relatively well, little is known about NS2. Antiserum was raised against a peptide near the N-terminus of NS2, and used to conduct Western blot analysis and immuno-fluorescence assays. Western blots revealed a prominent band near the expected size (41 kDa). Immuno-fluorescence studies of mosquito cells transfected with AeDNV indicate that NS2 has a wider distribution pattern than does NS1, and the distribution pattern appears to be a function of time post-infection. Nuclear localization of NS2 requires intact C-terminus but does not require additional viral proteins. Mutations ranging from complete NS2 knock-out to a single missense amino acid substitution in NS2 can significantly reduce viral replication and production of viable progeny.

SUBMITTER: Azarkh E

PROVIDER: S-EPMC2440674 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature

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Mosquito densonucleosis virus non-structural protein NS2 is necessary for a productive infection.

Azarkh Eugene E Robinson Erin E Hirunkanokpun Supanee S Afanasiev Boris B Kittayapong Pattamaporn P Carlson Jonathan J Corsini Joe J

Virology 20080128 1

Mosquito densonucleosis viruses synthesize two non-structural proteins, NS1 and NS2. While NS1 has been studied relatively well, little is known about NS2. Antiserum was raised against a peptide near the N-terminus of NS2, and used to conduct Western blot analysis and immuno-fluorescence assays. Western blots revealed a prominent band near the expected size (41 kDa). Immuno-fluorescence studies of mosquito cells transfected with AeDNV indicate that NS2 has a wider distribution pattern than does ...[more]

PMID: 18222517

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