Unknown

Dataset Information

0

Annexin A2 interactions with Rab14 in alveolar type II cells.

ABSTRACT: Annexin A2, a calcium-dependent phospholipid-binding protein, is abundantly expressed in alveolar type II cells where it plays a role in lung surfactant secretion. Nevertheless, little is known about the details of its cellular pathways. To identify annexin A2-regulated or associated proteins, we silenced endogenous annexin A2 expression in rat alveolar type II cells by RNA interference and assessed the change of the cellular transcriptome by DNA microarray analysis. The loss of annexin A2 resulted in the change of 61 genes. Thirteen of the selected genes (11 down-regulated and 2 up-regulated genes) were validated by real time quantitative PCR. When the loss of rat annexin A2 was rescued by overexpressing EGFP-tagged human annexin A2, six of seven selected targets returned to their normal expression level, indicating that these genes are indeed annexin A2-associated targets. One of the targets, Rab14, co-immunoprecipitated with annexin A2. Rab14 also co-localized in part with annexin A2 and lamellar bodies in alveolar type II cells. The silencing of Rab14 resulted in a decrease in surfactant secretion, suggesting that Rab14 may play a role in surfactant secretion.

SUBMITTER: Gou D 

PROVIDER: S-EPMC2442313 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Annexin A2 interactions with Rab14 in alveolar type II cells.

Gou Deming D   Mishra Amarjit A   Weng Tingting T   Su Lijing L   Chintagari Narendranath Reddy NR   Wang Zhixin Z   Zhang Honghao H   Gao Li L   Wang Pengcheng P   Stricker Heidi M HM   Liu Lin L  

The Journal of biological chemistry 20080310 19

Annexin A2, a calcium-dependent phospholipid-binding protein, is abundantly expressed in alveolar type II cells where it plays a role in lung surfactant secretion. Nevertheless, little is known about the details of its cellular pathways. To identify annexin A2-regulated or associated proteins, we silenced endogenous annexin A2 expression in rat alveolar type II cells by RNA interference and assessed the change of the cellular transcriptome by DNA microarray analysis. The loss of annexin A2 resul  ...[more]

Similar Datasets

Unknown Annexin A7 and SNAP23 interactions in alveolar type II cells and in vitro: a role for Ca(2+) and PKC.
| S-EPMC3432737 | biostudies-literature
Unknown Silver nanowire interactions with primary human alveolar type-II epithelial cell secretions: contrasting bioreactivity with human alveolar type-I and type-II epithelial cells.
| S-EPMC4765325 | biostudies-literature
Unknown Modeling of annexin A2-Membrane interactions by molecular dynamics simulations.
| S-EPMC5609761 | biostudies-literature
Unknown Dissecting the Niche for Alveolar Type II Cells With Alveolar Organoids.
| S-EPMC7287157 | biostudies-literature
Unknown Modulation of human type II secretory phospholipase A2 by sphingomyelin and annexin VI.
| S-EPMC1218659 | biostudies-other
Unknown Ca(2+)-dependent binding of annexin IV to surfactant protein A and lamellar bodies in alveolar type II cells.
| S-EPMC1136242 | biostudies-other
Unknown Alveolar epithelial type II cells activate alveolar macrophages and mitigate P. Aeruginosa infection.
| S-EPMC2654511 | biostudies-literature
Unknown Hemoglobin is expressed in alveolar epithelial type II cells.
| S-EPMC1314978 | biostudies-literature
Transcriptomics Role of annexin A2 in muscle repair
2016-10-04 | GSE87557 | GEO
Unknown Annexin A2 is regulated by ovarian cancer-peritoneal cell interactions and promotes metastasis.
| S-EPMC3787151 | biostudies-literature