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Use of laser microdissection for phylogenetic characterization of polyphosphate-accumulating bacteria.

ABSTRACT: Our novel approach for taxonomic identification of uncultured bacteria harboring specific physiological features in complex environmental samples combines cell collection by laser microdissection and subsequent DNA analysis. The newly developed approach was successfully tested for collection and phylogenetic characterization of polyphosphate-accumulating bacteria in activated sludge and lake sediment.

SUBMITTER: Gloess S 

PROVIDER: S-EPMC2446515 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature

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Use of laser microdissection for phylogenetic characterization of polyphosphate-accumulating bacteria.

Gloess Stefanie S   Grossart Hans-Peter HP   Allgaier Martin M   Ratering Stefan S   Hupfer Michael M  

Applied and environmental microbiology 20080502 13

Our novel approach for taxonomic identification of uncultured bacteria harboring specific physiological features in complex environmental samples combines cell collection by laser microdissection and subsequent DNA analysis. The newly developed approach was successfully tested for collection and phylogenetic characterization of polyphosphate-accumulating bacteria in activated sludge and lake sediment. ...[more]

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