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Immunomagnetic separation combined with real-time reverse transcriptase PCR assays for detection of norovirus in contaminated food.

ABSTRACT: We developed an immunomagnetic separation (IMS) technique combined with real-time TaqMan reverse transcriptase PCR (RT-PCR), which allowed detection of norovirus at a level as low as 3 to 7 RT-PCR units from artificially contaminated strawberries. The inoculum recovery rate ranged from 14 to 30%. The data demonstrate that IMS combined with real-time RT-PCR will be useful as a rapid and sensitive method for detecting food-borne microbial contaminants.

SUBMITTER: Park Y

PROVIDER: S-EPMC2446532 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature

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Immunomagnetic separation combined with real-time reverse transcriptase PCR assays for detection of norovirus in contaminated food.

Park YoungBin Y Cho You-Hee YH Jee YoungMee Y Ko GwangPyo G

Applied and environmental microbiology 20080425 13

We developed an immunomagnetic separation (IMS) technique combined with real-time TaqMan reverse transcriptase PCR (RT-PCR), which allowed detection of norovirus at a level as low as 3 to 7 RT-PCR units from artificially contaminated strawberries. The inoculum recovery rate ranged from 14 to 30%. The data demonstrate that IMS combined with real-time RT-PCR will be useful as a rapid and sensitive method for detecting food-borne microbial contaminants. ...[more]

PMID: 18441102

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