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Development of reverse transcriptase PCR assays for detection of active human herpesvirus 6 infection.

ABSTRACT: We developed reverse transcriptase (RT) PCR assays for the detection of mRNA from three spliced genes of human herpesvirus 6 (HHV-6), the immediate-early genes U16/U17 and U89/U90 and the late gene U60/U66. Sequence analysis determined the splicing sites of these genes. The new assays may be instrumental in investigating the association between HHV-6 and disease.

SUBMITTER: Van den Bosch G

PROVIDER: S-EPMC88132 | biostudies-literature | 2001 Jun

REPOSITORIES: biostudies-literature

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Development of reverse transcriptase PCR assays for detection of active human herpesvirus 6 infection.

Van den Bosch G G Locatelli G G Geerts L L Fagà G G Ieven M M Goossens H H Bottiger D D Oberg B B Lusso P P Berneman Z N ZN

Journal of clinical microbiology 20010601 6

We developed reverse transcriptase (RT) PCR assays for the detection of mRNA from three spliced genes of human herpesvirus 6 (HHV-6), the immediate-early genes U16/U17 and U89/U90 and the late gene U60/U66. Sequence analysis determined the splicing sites of these genes. The new assays may be instrumental in investigating the association between HHV-6 and disease. ...[more]

PMID: 11376078

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