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DNA detection and signal amplification via an engineered allosteric enzyme.

ABSTRACT: Rapid, sensitive, and sequence-specific DNA detection can be achieved in one step using an engineered intrasterically regulated enzyme. The semi-synthetic inhibitor-DNA-enzyme (IDE) construct (left) rests in the inactive state but upon exposure to a complementary DNA sequence undergoes a DNA hybridization-triggered allosteric enzyme activation (right). The ensuing rapid substrate turnover provides the built-in signal amplification mechanism for detecting approximately 10 fmol DNA in less than 3 min under physiological conditions.

SUBMITTER: Saghatelian A

PROVIDER: S-EPMC2453066 | biostudies-literature | 2003 Jan

REPOSITORIES: biostudies-literature

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DNA detection and signal amplification via an engineered allosteric enzyme.

Saghatelian Alan A Guckian Kevin M KM Thayer Desiree A DA Ghadiri M Reza MR

Journal of the American Chemical Society 20030101 2

Rapid, sensitive, and sequence-specific DNA detection can be achieved in one step using an engineered intrasterically regulated enzyme. The semi-synthetic inhibitor-DNA-enzyme (IDE) construct (left) rests in the inactive state but upon exposure to a complementary DNA sequence undergoes a DNA hybridization-triggered allosteric enzyme activation (right). The ensuing rapid substrate turnover provides the built-in signal amplification mechanism for detecting approximately 10 fmol DNA in less than 3 ...[more]

PMID: 12517141

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