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A single-molecule nanopore device detects DNA polymerase activity with single-nucleotide resolution.

ABSTRACT: The ability to monitor DNA polymerase activity with single-nucleotide resolution has been the cornerstone of a number of advanced single-molecule DNA sequencing concepts. Toward this goal, we report the first observation of the base-by-base DNA polymerase activity with single-base resolution at the single-molecule level. We describe the design and characterization of a supramolecular nanopore device capable of detecting up to nine consecutive DNA polymerase-catalyzed single-nucleotide primer extensions with high sensitivity and spatial resolution (

SUBMITTER: Cockroft SL 

PROVIDER: S-EPMC2453067 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature

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A single-molecule nanopore device detects DNA polymerase activity with single-nucleotide resolution.

Cockroft Scott L SL   Chu John J   Amorin Manuel M   Ghadiri M Reza MR  

Journal of the American Chemical Society 20080101 3

The ability to monitor DNA polymerase activity with single-nucleotide resolution has been the cornerstone of a number of advanced single-molecule DNA sequencing concepts. Toward this goal, we report the first observation of the base-by-base DNA polymerase activity with single-base resolution at the single-molecule level. We describe the design and characterization of a supramolecular nanopore device capable of detecting up to nine consecutive DNA polymerase-catalyzed single-nucleotide primer ext  ...[more]

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