Ontology highlight
ABSTRACT:
SUBMITTER: Manrao EA
PROVIDER: S-EPMC3757088 | biostudies-literature | 2012 Mar
REPOSITORIES: biostudies-literature
Nature biotechnology 20120325 4
Nanopore technologies are being developed for fast and direct sequencing of single DNA molecules through detection of ionic current modulations as DNA passes through a pore's constriction. Here we demonstrate the ability to resolve changes in current that correspond to a known DNA sequence by combining the high sensitivity of a mutated form of the protein pore Mycobacterium smegmatis porin A (MspA) with phi29 DNA polymerase (DNAP), which controls the rate of DNA translocation through the pore. A ...[more]